The several studies have consistently shown that low levels of plasmatic high-density lipoprotein (HDL) and high levels of low- and very low-density lipoprotein (LDL and VLDL, respectively) are linked with a sedentary lifestyle and are strong predictor to cardiovascular disease 1 2 3 4 5 . In addition, many of these diseases associated to sedentary lifestyle are also characterized by alterations in pro-inflammatory markers in the plasma 6 7 . These inflammatory process include increase in plasma the pro-inflammatory cytokines as tumor necrosis factor alpha (TNF-α), plasminogen activator inhibitor type-1 (PAI-1), C-reactive protein (CRP), interleukin 6 (IL-6) and reduction of anti-inflammatory cytokines as interleukin 10 (IL-10) and adiponectin 8 9 10 11 .

The plasmatic PAI-1 and CRP levels are also strongly related to cardiovascular risk factors, increasing its levels leading of arising the hypertension, high triglyceride levels and likely obesity 6 12 13 14 15 16 . It is now established that reduction in the plasma to adiponectin and increased PAI-1 and CRP levels play a role in the maintenance of an inflammatory state and in the development of cardiovascular disease 4 . However, it is much less known if these pro- and anti-inflammatory markers are related to the lipoproteins and cholesterol levels in plasma. While competitive exercise training programs (characterized by moderate/high intensity and long duration) seem to reduce the LDL and total cholesterol and to increase the HDL concentration, evicting the progression or appearance of inflammatory atherosclerotic process 6 , it remains slightly unknown if highly trained athletes have lower pro-inflammatory and higher anti-inflammatory markers than sedentary subjects.

Therefore, the objective of this study was to compare plasma levels adiponectin, PAI-1, CRP, and lipoprotein fractions between sedentary subjects and highly trained athletes. We hypothesised that individuals that performed high amount of exercise (moderate/high intensity and long duration) have improved lipid profile and inflammatory factors levels in relation sedentary subjects.

The subjects have similar height, body mass, and body mass index (table 1). The weekly exercise intensity, frequence, volume and time experience by highly trained athletes groups are also showed in Table 1.

Table 2 show plasmatic lipid profile, glucose, PAI-1, adiponectin, and CRP levels for the two groups. Was observed less plasmatic levels the Total cholesterol (p < 0.001), LDL-c (p < 0.01) and TG (p < 0.038) in highly trained athletes than in sedentary group. Plasmatic PAI-1 level was significantly lower (p < 0.0001) in highly trained athletes when compared with sedentary group. The plasmatic adiponectin (p < 0.88), CRP (p < 0.93), glucose (p < 0.10), LDL-oxidized (p < 0.09), HDL-c (p < 0.32) concentrations and Total Cholesterol/HDL-c ratio (p < 0.49) were not significantly different between the groups.

Was found a correlation positive between plasmatic PAI-1 and total cholesterol level (r = 0.78, p = 0.0009). The plasma PAI-1 level was also positively associated with LDL-c (r = 0.69, p = 0.006) and TG (r = 0.56, p = 0.03). All correlation is showed in Figure 1. No significant correlations were found between PAI-1 and any other variable. No correlations were found between adiponectin and CRP with any variable (p > 0.05). The volume and exercise frequency of the highly trained athletes group was inversely associated with TG concentration (r = -0.76 and -0.77, respectively; p < 0.05; data not showed).

The results of the present study indicate that, highly trained athletes subjects that perform high amount of the exercise shown lower plasmatic lipid profile and PAI-1 levels than sedentary subjects. In addition, the plasma total cholesterol, LDL-c and TG concentration were positively correlated with pro-inflammatory PAI-1 levels.

The data of the present study confirm previous findings that subjects trained have lower plasmatic TG, LDL-c and total cholesterol concentration than sedentary subjects 20 21 . Tsekouras et al 22 examined the effect of high intensity intervals of aerobic training on VLDL-TG secretion in men. They observed that subjects who had trained by running on the treadmill for 8 weeks at 90% VO_2peak had a reduced rate of VLDL-TG secretion, suggesting that subjects that perform high intensity exercise induced changes in plasmatic lipid profile. Moderate chronic exercise also induces an augment of lipase lipoprotein gene expression and activity, both in the skeletal muscle and the adipose tissue 23 24 , resulting in decreased plasma TG content and liver VLDL output 25 26 . Additionally, aerobic exercise increase the activity of lecithin:cholesterol aciltransferase (L-CAT), the enzyme responsible for cholesterol ester transfer to HDL-c and reduces the activity of the plasmatic cholesterol ester transfer protein (CETP), the enzyme responsible for transferring the ester in HDL cholesterol to other lipoproteins 27 28 29 30 31 . These alterations may lower the concentrations of total cholesterol and LDL-c in the plasma through the exchange of cholesterol ester between tissues and lipoproteins to HDL-c 32 .

The plasmatic PAI-1 levels were less in highly trained athletes in relation to sedentary subjects, as showed in Table 1. The fibriolytic system is a proteolytic enzyme system with many physiological functions of which degradation of fibrin deposits in blood vessels is the best known and possibly the most important. Studies have indicated that reduced fibrinolytic capacity, mainly due to elevated plasma levels of PAI-1, may have pathogenetic importance in myocardial infarction, particularly in patients with hypertriglyceridemia 33 . Páramo et al 4 , observed a significant reduction in PAI-1 activity after aerobic regular long-term (9 months duration) indicating that exercise training had beneficial effect on fibrinolysis. Stratton et al 34 also showed that exercise training enhances fibrinolysis in healthy older men by increasing resting levels of tissue plasminogen activator and decreasing PAI-1 activity. The results of the present study indicated that high amount of exercise reduces PAI-1 levels in plasma.

Sedentary lifestyle increases the risk of developing cardiovascular disease and diabetes 1 , and many other diseases which are linked to the inflammatory markers in the plasma 7 . Patients with elevated plasma cholesterol and with coronary atherosclerosis have increased plasma levels of PAI-1 35 . Some studies indicate that this may be a consequence of the endothelial dysfunction caused by high plasma lipoprotein levels, and it is assumed that this is one of the principal causes by which these patients are at higher risk for myocardial infarctions 36 . The results of the present study comparing highly trained athletes with sedentary subjects indicates that high amount of exercise exert an important protector effect for reduced both lipid profile and PAI-1 levels.

A positive and fairly strong relationship between triglycerides, LDL, total cholesterol and PAI-1 levels in plasma is of particular interest, since it raises the possibility that hypertriglyceridemia is connected with a predisposition to thrombosis through a coexisting increase in PAI-1 concentration 33 . Jovin et al 35 related that, plasma LDL can induce an increased release of PAI-1 by endothelial cells into the vessel lumen and can contribute to the release of PAI-1 into the subendothelial space and thus to the process of atherosclerotic plaque remodelling and rupture. The significant correlations found in the present study between PAI-1 and TG, LDL and total cholesterol levels suggest that the elevated pro-inflammatory process in sedentary group may be due to the elevated values of the lipids in plasma.

In present study, no differences were verified in relation plasmatic CRP and adiponectin levels between sedentary and highly trained athletes. Changes in plasmatic CRP and adiponectin levels are more observed in weight loss interventions 5 6 37 38 . These data indicate that these adipokines may not be good indicators of processes linked to the increase in the lipid profile in subjects with sedentary lifestyle.

The limitations of this study include the relatively small numbers of subjects in each of the groups and the lack of subjects with cardiovascular and other diseases in which the changes in variables are assessed. Future studies should address the ability of exercise training to alter fibrinolytic variables in these groups and should also evaluate the effects of longitudinal training protocols.

In summary, our results indicate that sedentary subjects present a favourable state leading to hypertriglyceridemia and hypercholesterolemia accompanied by high PAI-1 levels. In contrast, highly trained athletes performance and high amount of the exercise reduced greatly the possibility of appearance of cardiovascular diseases.

The authors declare that they have no competing interests.

FSL, JCR, AELS, HAS, ECC, MCS, ARD, LMO and RVTS participed the sample collected, assess samples, design of the study and performed the statistical analysis, and writing of paper. All authors read and approved the final manuscript